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KMID : 0613820040140040644
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Journal of Life Science
2004 Volume.14 No. 4 p.644 ~ p.649
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Antisense GA 3β-Hydroxylase Gene Transferred to Rice Plants
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Kang Yong-Won
Yoon Yong-Hwi
Kim Kil-Woong
Lee In-Jung
Shin Dong-Hyun
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Abstract
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During plant development, active gibberellins (GAs) control many aspects of plant growth and development including seed germination, stem elongation, flower induction, anther development and seed growth. To understand the biosynthesis and functional role of active GAs in high plants, this study investigated GA 3β-hydroxylase gene encoding GA1 and GA4 catalizing last step in GA biosynthetic pathway. The antisense GA 3β-hydroxylase gene was inserted into expression vector, pIG121-Hm. Calli derived from mature seeds of rice (Oryza sativa L. cv. Donjinbyeo) were co-cultivated with Agrobacterium tumefaciens EHA101 carring a pIG121-Hm containing hygromycin resistance (Hygr) and antisense GA 3β-hydroxylase gene. Seventeen transgenic plants obtained inhibiting GA 3β-hydroxylase. Transgenic plants had shorter plant height more than that of the Dongjinbyeo. Stable integration of antisense GA 3β-hydroxylase gene was confirmed by polymerase chain reaction of genomic DNA isolated from the leaf organs of the T0 generation.
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KEYWORD
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Antisense GA 3β-hydroxylase, Polymerase chain reaction, Rice (Oryza sativa L.), Transgenic plants, T0 generation
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